Comparison Of Low Polar Volatile Components From Supercritical Co_2 Extracts From Roots And Stems Of Gangmei And Their Effects On Proliferative Activity Of Iec-6 Cells
To compare the effects of low polar volatile constituents from supercritical CO2 extracts from roots and stems of Gangmei on the proliferation of IEC-6 cells in rat small intestinal recess in vitro, so as to provide reference for making full use of wild resources of Gangmei and expanding its medicinal parts. METHODS: The low polar volatile constituents from the roots and stems of Prunus officinalis were extracted by supercritical CO2 extraction and analyzed by gas chromatography-mass spectrometry (GC-MS). IEC-6 cells were treated with different concentrations of supercritical CO2 extracts (0, 1, 5, 10, 20, 40, 60, 80, 100 ug/mL) from roots or stems of Prunus officinalis. Cell viability was measured by MTT method. Cell proliferation curve was drawn and half of the effective concentration (EC50) of each extract was calculated. RESULTS: 62 and 46 low polar volatile compounds were identified from the supercritical CO2 extracts of the roots and stems of Gangmei by GC-MS, of which 24 were mainly NONYLIC acid (14.18% and 6.14% respectively in roots and stems), octanoic acid (10.59%, 4.35% in roots and stems), hexanoic acid (8.63%, 10.86% in roots and stems), paeonol (7.79% in roots and stems, respectively). 6.00%, 2-methyl-3-phenyl-propanol (6.30% and 0.58% in root and stem respectively), acetic acid (1.72% and 33.77% in root and stem respectively). The results of cell culture in vitro showed that the cell proliferation of IEC-6 was significantly promoted when the concentration of supercritical CO2 extracts from roots and stems was lower (<60 ug/mL). EC50 was 16.35 and 20.20 ug/mL, respectively. When the concentration of supercritical CO2 extracts was higher (>80 ug/mL), the cells showed cytotoxic activity and inhibited the proliferation of IEC-6 cells. CONCLUSION: The low polar volatile constituents in the roots and stems of Prunus officinalis are similar, and their extracts have similar bioactivities to IEC-6 cells in vitro. Short-chain fatty acids may be the active ingredient to promote cell proliferation, while paeonol may be the cytotoxic active ingredient.
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