Study on Supercritical Extraction of Volatile Oil from Radix Notopterygii and Its Inhibition of Tyrosinase Activity
The extraction process of volatile oil from Radix Notopterygii was optimized by supercritical fluid extraction, and its whitening activity was studied. On the basis of single factor experiment and taking the yield of extract as the evaluation index, the optimum extraction process was determined by orthogonal test and the principle of time saving and economy. L-DOPA was used as substrate to determine the inhibitory rate of tyrosinase from volatile oil of Radix Notopterygii, investigate its whitening activity, draw the Lineweaver-Burk curve and infer its inhibitory type. The optimum extraction conditions of volatile oil from Radix Notopterygii were determined by experiments as follows: extraction pressure 25 MPa, extraction temperature 45 C and CO2 gas flow rate 28 L.h-1. Under this condition, the yield of volatile oil was 8.91%. The volatile oil of Radix Notopterygii has good tyrosinase inhibitory activity. Its half inhibitory concentration (IC50) is 1.36 g/m L, and the inhibitory type is mixed. Supercritical extraction method can be used to extract volatile oil from Radix Notopterygii with high extraction rate. The obtained volatile oil from Radix Notopterygii has good inhibitory effect on tyrosinase.
Key words: Radix Notopterygii CO2 Extraction, Volatile Oil Extraction, Supercritical Extraction