Supercritical CO2 Extraction of Cannabinol from Hemp and Its Antibacterial Activity
Supercritical CO2 extraction of cannabinol from hemp was established, and the antimicrobial properties of the extracts were investigated. Through the analysis of single factor and orthogonal test, the optimum extraction process of three cannabinoids from hemp by supercritical CO2 was determined: extraction temperature 45 C, extraction time 70 min, entrainer dosage 300 m L, extraction times 3 times. The bacteriostasis tests of E. coli and Staphylococcus aureus with MIC of 8 mg/ml and 6 mg/ml were carried out by filter paper method. Under these conditions, cannabinol (CBD) was 3.189 mg/g, hexahydrocannabinol (CBN) was 2.206 mg/g, tetrahydrocannabinol (9-THC) was 58.757 mg/g, that is, the three cannabinoids were 64.152 mg/g. The diameter of bacteriostasis circle of Escherichia coli and Staphylococcus aureus were 15.4 mm and 25.8 mm, respectively. The supercritical extract had certain bacteriostasis.
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